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Chemicals and reagents 4. Cell cycle analysis by flow cytometry 4. Immunoblot Results 4.
Increases in p53 levels can also lead to increased expression of numerous p53 target genes and one of the most importan is cyclin-dependant kinase inhibitor A (CDKN1A) or p21. Cdk inhibitor p21 (p21CIP1) is also regarded as a downstream effector gene (Pellegata et al 1996). Human p21 gene located at chromosome 6 can act as a regulator for cell cycle progression controlled by p53 (Gartel and Radakrishnan 2005). Thus the positive links between p53 and its effector gene p21 lead to binding of p21 to Cyclin-Cdks complexes which in turn inhibit the cells in G1 phase (Morgan 2007). Structural organisation of p53 protein. The p53 393 amino acids comprise five main domains including acidic N-terminal region containing the transactivation domain and mdm2 binding site (1-50) a proline rich domain
(6392) a central domain cotaining the sequence-specific DNA-binding domain (100-300) and c-terminal or tetramerisation domain consists of the oligomerisation domain (323-358) containing nuclear export signal and the regulatory domain (363-393) containing the Using Kratom To Quit Smoking nuclear localisation signals a Using Kratom To Quit Smoking smoking kratom dosage nonspecific DNA binding domain that bind to damaged DNA and act as negative regulator of DNA Using Kratom To Quit Using Kratom To Quit Smoking Smoking binding of the central domain. November 2003 Cambridge University Press.
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