Possible involvement of pro-apoptotic caspases (8 and 9) The caspase 8 colorimetric assay performed on SH-SY5Y cell lysates indicated little difference between all MSE treated groups and control group for both 4 hr and 24 hr incubation time period (Fig. Malaysian Kratom Powder Wedgefield a and B). The
same outcome was also noted for caspase 9 assay which was performed using the same cell lysates (Fig. C and D). At the 24 hr time point of both caspase assays (Fig.
Effect of MIT on cell cycle distribution of SH-SY5Y cells after 24 hr treatment. Histograms and values of the cell cycle phases are representative of a single experiment analysed by Modfit software. Protein concentrations of the cell lysates The bicinchoninic assay (BCA) is quick and works in a similar way to the Lowry method. Smith et al 1985). It is one of the recommended assays for determining protein content of cell lysates used for gel electrophoresis in immunoblotting. BCA protein assay kit (Fig. Routinely BSA calibration curves were used to determine the protein concentrations in SHSY5Y cell lysates.
Discussion Mitragyna speciosa Korth (Kratom) leaves have been used by humans for decades. There are no reports of increased cancer associated with Malaysian Kratom Powder Wedgefield consumption of Kratom leaves although such associations have never been examined in a proper controlled study. Neither is there any information available concerning the genotoxic potential of
Kratom leaves. As part of
establishing a database on the toxicological potential of the use of this plant I have attempted to examine the possible toxicological effects this plant might have including potential for carcinogenicity via genotoxicity testing. The basic toxicology data established in the previous chapter has informed us on the potential cytotoxicity of MSE and MIT on several human cell lines which generally shows cytotoxicity with high dose. The lethal effect of the extract and major kratom powder where to buy alkaloid (MIT) on the cells examined kratom pills online prompted the question whether cell death was accompanied kratom withdrawal restlessness by DNA damage.