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The bacterial tryptophan reverse mutation assay with Escherichia coli WP2. Rapid colorimteric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Immunol Methods 65: 5563.
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Surprisingly this time a similar outcome was observed for both SH-SY5Y and MCL-5 cells and the shifting of the whole populations was evident at much lower concentrations of MSE than in the previous PI staining in chapter 2. This phenomenon is obviously due to the treatment effects as the control and lowest concentration of the MSE tested as seen in fig. The hypothesis of plasma membrane opening is supported with this finding. This phenomenon creates disadvantages for this assay as when the whole FACS profile shifts to kratom cranberry juice east brunswick the right side of the scale the determination of the stages of cell death is difficult to interpret as the cells are no longer located in specific quadrants. This observation is clearly in contrast with the previous cytological kratom herbal supplements examinations which indicated that SH-SY5Y cells treated with high dose of MSE undergo apoptosis rather
The other concentrations tested were negative for genotoxic potential. The presence of S9 appeared to have a substantial effect on the RTG with MSE. In fact there was a clear dose-dependant toxicity Kratom Underground Reddit observed suggesting that the MSE was being activated to a toxic derivatives.
The cytological examinations performed previously indicated that SH-SY5Y cells treated with MSE commit to death predominantly via apoptosis especially at high dose of MSE. MSE appeared to have little effect compared to control group and shows similar profile in terms of distribution of percentages of four quadrants. Interestingly at higher MSE concentration the profile of the four different populations was drastically changed as the whole population shifted to the right side of the scale. This finding is consistent with the result of the previous flow cytometry analysis with PI staining performed in chapter 4 section 4. For MIT treated cells changes of the four populations were not as drastic as MSE treated cells. Q3 and Q4 indicating increased of apoptotic is kratom bad for you pen argyl and necrotic cells. For MCL-5 cells (Fig 5.