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The kratom powder microwave first bottom left quadrant (Q1) represent the live cells which exclude both stains (Annexin V and 7-AAD) the top left quadrant (Q2) represent the Annexin V positive cells indicating early Indo Bomb Kratom Wendel apoptosis population the top right quadrant (Q3) represents the Annexin V and 7-AAD positive cell population indicating necrosis and the last bottom right quadrant (Q4) represents the 7-AAD positive cell population indicating late stage of apoptosis population. The cytological examinations performed previously indicated that kratom capsules good SH-SY5Y cells treated with MSE commit to death predominantly via apoptosis especially at high dose of MSE. MSE appeared to have little effect compared to control group and shows similar profile in termsof distribution of percentages of four Indo Bomb Kratom Wendel
quadrants. Interestingly at higher MSE concentration the profile of the four different populations was drastically changed as the whole population shifted to the right side of the scale.
SH-SY5Y cells treated with chloroform in ethanol vehicle (Fig. If chloroform contamination of MSE contributed to the toxicity of MSE then buy kratom dallas addition or synergistic cytotoxicity would be expected. M CHCl3) (Fig. This result suggests that chloroform did not enhance MSE-dependant cytotoxicity. C 5 o 1. MS E .
P53 levels of MIT treated SH-SY5Y cells after 24 hr treatment. P53 levels of MIT treated SH-SY5Y cells at different time points (6 12 24 and 48 hr). Effects of MSE and MIT on p53 target gene product p21 It is well established that induction of p53 can lead to expression of target gene p21 and thereby cell cycle arrest. MSE even at the earliest time point 6 hr. Therefore
to further determine whether p21 is positively linked with p53 in response to MSE or MIT we examined p21 levels using immunoblots.