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<p>The mutant frequency value was determined <b>Buy Kratom In Oklahoma Seibert</b>  from the derived number of mutant colonies in medium containing TFT and the number of colonies growing in nonTFT medium. The preliminary data on selection of dose range and final summary of the MLA results for the MSE and MIT <a href=>are</a> discussed below: 3. MLA for MSE As shown in table 3.</p>
<p>C (5% CO2) for 48 hr time period. After incubation the cells were harvested and trypsinised as described in chapter 2 section 2. The cell pellets were then prepared for flow cytometry analysis using PI staining as described in chapter 4 section 4. The cells stained with PI were analysed using BD FacsCaliburflow cytometer. PI was excited at 488 nm and </p>
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experiments were also carried out for MIT as shown in fig. There was no significant difference in the p53 levels noted over the dose range used however they appeared to be down regulated compared to the control group. The time course of MIT induced p53 change was also carried as shown in fig. M MIT indicating the loss of p53 protein over time. The findings described above suggest that the cell cycle arrest of MSE treated cells seen previously with flow cytometry was independent of p53 protein induction and to the lesser extent for MIT kratom legal status by state treated cells.