Prior to this Botanical Wellness Kratom study MIT was thought to be the compound responsible for the narcotic effects of this plant. In the early part of this study basic in vitro
toxicology revealed that MSE and MIT have dose dependant toxicity to several human cell lines and the SH-SY5Y cell was the most sensitive. Botanical Wellness Kratom this is not surprising as the central nervous system was pharmacologically determined as the target system for the biological effects of this plant thus a toxicity response might be anticipated in neuronal cells. In the present study it is suggested that the toxicity effects seen for MSE were predominantly due to MIT as shown by similar IC50 values for MIT and MSE treated SH-SY5Y cells. The role of metabolism was also assessed in which the toxicity of kratom 15x tea williamsport MSE treated SH-SY5Y cells was found to kratom jacksonville fl blooming prairie increase 10-fold when the metabolic Botanical Wellness Kratom activation system post mitochondrial rat liver S9 induced by Arochlor 1254 Botanical Wellness Kratom was added to the treatment. However contradictory results were noted
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metabolically competent MCL-5 cells appeared to detoxify MSE rather than activate it. S9 that contribute to activating MSE toxicity.
C and D). At the 24 hr time point of both caspase assays (Fig. Activity of initiator caspase 8 after A) 4 hr incubation and B) 24 hr incubation mitragyna speciosa suppliers time period and initiator caspase 9 after C) 4 hr incubation and D) 24 hr incubation time period of SH-SY5Y cells treated with MSE. The reading of each concentration is from 2 pooled lysates. SH-SY5Y cells treated with high dose of MSE and MIT incubated for 4 and 18 hrs respectively as described in the section 5. As shown in fig. A there was a non-significant difference noted for caspase 3 and 7 activities for MSE treated cells compared to control Botanical Wellness Kratom Botanical Wellness Kratom groups at 4 hr
incubation time point.