Tris 2 g SDS in 500 ml distilled water pH 8. Sources and dilutions of primary and secondary antibodies for p53 and p21 protein used for the immunoblot assay. Behavioral And Neurochemical Characterization Of Kratom (mitragyna Speciosa) Extract the DNA profiles of three different cell lines (HEK 293 MCL-5 and SH-SY5Y cells) treated with MSE and MIT were assessed using nucleic acid staining with PI and analysed with BD FacsCalibur flow cytometer in the Centre for Molecular Microbiology and Infection (CMMI) core facility unit Flowers Building South Kensington Campus.
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